Particle Size Determination

Related topics:

Dynamic light scattering is known as a fast and efficient screening method for aggregate / particle detection. Therefore, it is widely used within biopharmaceutical development. It is used to close the gap between results gained in the range of small soluble aggregates via size exclusion chromatography (SEC) and particle methods such as light obscuration (LO) or micro flow imaging (MFI). Due to the high sensitivity toward large species, even small impurities can be detected within the measurement range (0.5 nm – 1 µm). For more information please follow the link.

Asymmetrical flow field-flow fractonation is an orthogonal method to size exclusion chromatographie (SEC) and sedimentation velocity analytical ultracentrifugation (SV-AUC). AF4 can be used for a wide range of applications ranging from the detection of soluble aggregates, characterization of species via multi angle laser light scattering (MALLS) and determination of particle size distributions. For more information please follow the link.

Sedimentation velocity analytical ultracentrifugation (SV-AUC) is seen as the gold standard for aggregation analytics. The importance of SV-AUC results is growing especially in biosimilarity studies as well as the development of gene therapy products.   ZentriForce Pharma offers SV-AUC services for a wide range of samples. For more information please follow the link.

The detection of soluble aggregates is a crucial step for a wide range of bio-pharmaceutical products.The content of soluble aggregates is one of the most crucial quality attributes of pharmaceutical drug products. Their quantification is necessary for all drug products independent of the type of API. ZentriForce Pharma offers a wide range of analytical techniques for the detection of soluble aggregates.  If you need further information please follow the link

Depending on the size of your API particle size determination (between 1 nm and 1 μm ) can be one of the most important steps for the monitoring of your drug products quality. ZentriForce Pharma offers a wide range of techniques to support  (between 1 nm and 1 μm ). If you need further information please follow the link

ZentriForce Pharma offers a wide range of analytical techniques. Please follow the link to our analytical portfolio. 


General topics:

ZentriForce Pharma was founded as a service provider that specializes in hydrodynamic techniques for biopharmaceutical development. The focus lies on gene therapy products and biosimilarity studies. Follow the link to our company page for further information.

Please follow this link to our contact page

ZentriForce Pharma implemented a strict quality system to assure the highest tractability of results generated at our facility. We have a wide range of quality related standard operation procedures (SOPS) in place to assure the consistency and quality of our laboratory operations. Please follow the link to a description of our quality system. 

ZentriForce Pharma is your expert in analytical services, with focus on hydrodynamic techniques. Please follow the link to find out about our analytical services. 

ZentriForce Pharma offers a wide range of analytical techniques. Please follow the link to our analytical portfolio. 

ZentriForce Pharma is not merely an expert in analytical development. Our scientist are actively involved in further advances in the theory of hydrodynamic techniques. Follow the link to a detailed description of the theory behind our analytical services, including some of our own research

ZentriForce Pharma is always looking for motivated employees. Check out our career page for further information. 

Particle Size Determination 

Depending on the type of your API, particle size determination may be one of the most important steps for the monitoring of your drug product’s purity. For gene therapy products especially, it is important to analyze the particle size distribution. Our laboratory offers the analytical techniques that you need for the determination of particle size distributions. Below, we provide a portfolio of techniques, including their applicable size ranges. 

Dynamic Light Scattering (1 nm – 1 μm)

With the DynaPro®Plate Reader III ZentriForce Pharma offers highest quality DLS measurements. The instrument performs dynamic light scattering (DLS) as well as static light scattering (SLS) measurements. Its properties allow for drastic reduction of sample volume, with a minimum of 4 µL, without the need to introduce an oil film to cover the sample. Additionally, sample measurement at low concentrations is feasible, owing to the instrument’s high sensitivity.

Additionally, the instrument is equipped with a well-plate system that allows high throughput campaigns of up to 1536 samples in a single run. We can therefore assess the particle size distribution of different samples simultaneously, for example within a formulation development.

  • Measured values:
    • hydrodynamic radius / diffusion coefficient
    • polydispersity (PDI)
    • light scattering signal 
    • molecular weight

Asymmetrical Flow Field-Flow Fractionation (1 nm – 1 μm)

Asymmetrical flow field-flow fractionation (AF4) is an excellent technique for the determination of particle sizes in the considerable size range of 1 nm to 1 µm. AF4 is a first principle method that determines the hydrodynamic radius.). The combination of the fractionation method itself (AF4) with the use of UV, RI and MALLS detectors allows us to get the full picture about the measured samples. 

  • Measured values:
    • hydrodynamic radius / diffusion coefficient 
    • radius of gyration 
    • molecular weight 
    • UV signals at two wavelength 
    • RI signal
    • light scattering signal 

Analytical Ultracentrifugation (0.5 nm – 200 nm)

Analytical Ultracentrifugation (AUC) is an established technique for the determination of particle size distributions. It was originally developed for this very purpose nearly 100 years ago. Its properties are especially interesting for gene therapy products. AUC can separate species by size as well as density. This unique property gives access to information on sample purity (it detects fragments and aggregates) and on the ratio of particles that contain DNA to empty particles within a single measurement (applicable to, e.g., AAVs). 

  • Measured values:
    • sedimentation coefficient
    • molecular weight 
    • shape information
    • UV signals (multi-wavelength)
    • RI signal 

Dynamic light scattering is known as a fast and efficient screening method for aggregate / particle detection. Therefore, it is widely used within biopharmaceutical development. It is used to close the gap between results gained in the range of small soluble aggregates via size exclusion chromatography (SEC) and particle methods such as light obscuration (LO) or micro flow imaging (MFI). Due to the high sensitivity toward large species, even small impurities can be detected within the measurement range (0.5 nm – 1 µm). For more information please follow the link.

Asymmetrical flow field-flow fractonation is an orthogonal method to size exclusion chromatographie (SEC) and sedimentation velocity analytical ultracentrifugation (SV-AUC). AF4 can be used for a wide range of applications ranging from the detection of soluble aggregates, characterization of species via multi angle laser light scattering (MALLS) and determination of particle size distributions. For more information please follow the link.

Sedimentation velocity analytical ultracentrifugation (SV-AUC) is seen as the gold standard for aggregation analytics. The importance of SV-AUC results is growing especially in biosimilarity studies as well as the development of gene therapy products.   ZentriForce Pharma offers SV-AUC services for a wide range of samples. For more information please follow the link.

The detection of soluble aggregates is a crucial step for a wide range of bio-pharmaceutical products.The content of soluble aggregates is one of the most crucial quality attributes of pharmaceutical drug products. Their quantification is necessary for all drug products independent of the type of API. ZentriForce Pharma offers a wide range of analytical techniques for the detection of soluble aggregates.  If you need further information please follow the link

Depending on the size of your API particle size determination (between 1 nm and 1 μm ) can be one of the most important steps for the monitoring of your drug products quality. ZentriForce Pharma offers a wide range of techniques to support  (between 1 nm and 1 μm ). If you need further information please follow the link

ZentriForce Pharma offers a wide range of analytical techniques. Please follow the link to our analytical portfolio. 

Interested in Particle Size Determination Services ?

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