DLS Services for Virus Development

Related topics:

Dynamic light scattering is known as a fast and efficient screening method for aggregate / particle detection. It is widely used within biopharmaceutical development. DLS closes the gap between techniques like size exclusion chromatography (SEC), that measure small soluble aggregates and particle methods such as light obscuration (LO) or micro flow imaging (MFI). Due to a high sensitivity toward large species, even small impurities can be detected within the measurement range (0.5 nm – 1 µm). Please follow the link for more information.  

Sedimentation velocity analytical ultracentrifugation (SV-AUC) has the potential to become one of the most crucial techniques for quality evaluation of gene therapy products. Owing to their considerable size, intact viruses pose a challenge in analytics. However, our scientists have already tackled this problem before and use their experience to provide you with a custom-tailored method that is applicable to your complex samples. Please follow the link for more information.

Asymmetrical flow field-flow fractionation (AF4), coupled with a multiangle laser light scattering detector (MALLS), has the potential to develop into a crucial technique for the evaluation of virus product purity. It is already one of the main assays in several cases of gene therapy product development. For further information please follow the link

Light scattering is a well defined effect. The physical properties of light scattering are used for several applications, such as dynamic and static light scattering. Please follow the link to a detailed description of light scattering theory. 

We are offering a wide range of analytical techniques for the determination of particle size distributions.  The detection of nano particle size distributions (1 nm  – 1 μm) is growing in importance. Especially, since the interest in gene therapy increased. Our scientist are working since more than 10 years on the topic of particle size determination. Please follow the link for more information.

In the last couple of years, the interest in the determination of predictive parameters within formulation development has grown. Interactions parameters provide valuable information for the prediction of molecular stability. These values are used in a “design of experiment” (DOE) approach to assist formulation developments. Please follow the link for more information.


General topics:

ZentriForce Pharma was founded as a service provider that specializes in hydrodynamic techniques for biopharmaceutical development. The focus lies on gene therapy products and biosimilarity studies. Follow the link to our company page for further information.

Please follow this link to our contact page

ZentriForce Pharma implemented a strict quality system to assure the highest tractability of results generated at our facility. We have a wide range of quality related standard operation procedures (SOPS) in place to assure the consistency and quality of our laboratory operations. Please follow the link to a description of our quality system. 

ZentriForce Pharma is your expert in analytical services, with focus on hydrodynamic techniques. Please follow the link to find out about our analytical services. 

ZentriForce Pharma offers a wide range of analytical techniques. Please follow the link to our analytical portfolio. 

ZentriForce Pharma is not merely an expert in analytical development. Our scientist are actively involved in further advances in the theory of hydrodynamic techniques. Follow the link to a detailed description of the theory behind our analytical services, including some of our own research

ZentriForce Pharma is always looking for motivated employees. Check out our career page for further information. 

Dynamic Light Scattering (DLS) Services for Virus Development

Dynamic light scattering is an established analytical technique in the field of gene therapy product development. The size range of virus-based APIs (ca. 20-500 nm) lies well within the detection range of DLS (0.5 nm – 1 μm).

Dynamic light scattering is used to collect particle size distributions. The technique is robust and offers fast screening of large numbers of samples.  

With our state-of-the-art instrumentation we apply dynamic light scattering for the determination of particle size distributions and are able to additionally provide information on thermal stability. 

Please follow the link to our light scattering theory page to learn more about dynamic and static light scattering theory.

DLS Instrumentation

Our scientists have worked with a wide range of gene therapy products. Our experience in DLS method development keeps your expenses low.

With the DynaPro®Plate Reader III ZentriForce Pharma offers highest quality DLS measurements. The instrument performs dynamic light scattering (DLS) as well as static light scattering (SLS) measurements. Its properties allow for drastic reduction of sample volume, with a minimum of 4 µL, without the need to introduce an oil film to cover the sample. Additionally, sample measurement at low concentrations is feasible, owing to the instrument’s high sensitivity. The instrument’s well-plate system allows high throughput campaigns. This allows us to assess the particle size distribution of different samples, for example within a formulation development. 

DLS related Services

Simultaneous DLS and SLS (static light scattering) measurements enable us to combine information on particle size distribution and molecular weight. This data provides valuable information on stability. An example for the benefit of this method is the simultaneous determination of the second osmotic virial coefficient (A22) and the concentration dependence of the diffusion coefficient (kD). The combination of these two parameters is especially interesting within formulation development studies.  For further information follow this link

We additionally offer temperature-controlled measurements with the options to either ramp the temperature to access the aggregation temperature, or to monitor the stability at a constant temperature. The well-plate-based design allows us to measure the samples simultaneously instead of consecutively (as is the case in injection-chamber based setups).  We can therefore provide identical measurement conditions for all samples.  Please follow this link for further information.

Dynamic light scattering is known as a fast and efficient screening method for aggregate / particle detection. Therefore, it is widely used within biopharmaceutical development. It is used to close the gap between results gained in the range of small soluble aggregates via size exclusion chromatography (SEC) and particle methods such as light obscuration (LO) or micro flow imaging (MFI). Due to the high sensitivity toward large species, even small impurities can be detected within the measurement range (0.5 nm – 1 µm). For more information please follow the link.

Owing to their considerable size, intact viruses pose a challenge in analytics. However, our scientists have already tackled this problem before and use their experience to provide you with a tailored method that is applicable to complex samples.  ZentriForce Pharma is your partner for the development of a robust and specific SV-AUC method. Please follow the link for more information.

Asymmetrical flow field-flow fractonation (AF4), coupled with a multi-angle laser light scattering detector (MALLS) has the potential to develop into a crucial technique for the evaluation of virus product purity. It is already one of the main assays within several cases of gene therapy product development. For further information please follow the link

AUC is a first principle method. Therefore, the separation mechanism is well understood and extensively described. Please follow the link to a detailed description of AUC theory. 

We are offering a wide range of analytical techniques for the determination of particle size distributions.  The detection of nano particle size distributions (1 nm  – 1 μm) is growing in importance. Especially, since the interest in gene therapy increased. Our scientist are working since more than 10 years on the topic of particle size determination. Please follow the link for more information.

Interested in DLS Services ?

We are more than happy to assist you with our DLS services.

Dynamic Light Scattering Specifications

Main outputSize distribution (rH or dH); molecular weight (Mw)
Size range0.5 – 1000 nm; 1 kDa to 1 MDa
Additional outputsTemperature stability (Tonset, Tm, Tagg); Interaction parameter (A22, kD)
Sample volume4 µL to 150 µL
Measurement typewell plate (96, 384 or 1536 wells)
Temperature range 4°C to 85°C
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