Interaction Parameter Determination

Related topics:

Dynamic light scattering is known as a fast and efficient screening method for aggregate / particle detection. It is widely used within biopharmaceutical development. DLS closes the gap between techniques like size exclusion chromatography (SEC), that measure small soluble aggregates and particle methods such as light obscuration (LO) or micro flow imaging (MFI). Due to a high sensitivity toward large species, even small impurities can be detected within the measurement range (0.5 nm – 1 µm). Please follow the link for more information.  

Sedimentation velocity analytical ultracentrifugation (SV-AUC) is the gold standard in aggregation analytics. The importance of SV-AUC results is growing, especially in biosimilarity studies, and also within the development of gene therapy products.   ZentriForce Pharma offers SV-AUC services for a wide sample spectrum. For more information please follow the link.

Light scattering is a well defined effect. The physical properties of light scattering are used for several applications, such as dynamic and static light scattering. Please follow the link to a detailed description of light scattering theory. 

Sedimentation velocity analytical ultracentrifugation (SV-AUC) method is the established standard experiment when it comes to AUC. It is carried out at high rotational speed and the easiest way to obtain sample composition and species characteristics (e.g. molecular weight and frictional ratio). Follow this link to get more information about SV-AUC theory.

While sedimentation velocity is the standard AUC experiment, sedimentation equilibrium analytical ultracentrifugation (SE-AUC) nonetheless offers valuable information on an analyte.  SE-AUC is a first principle method for the determination of the molecular weight. Therefore, it is the method of choice to determine the molecular weight of your API in solution. Follow this link to get more information about SE-AUC theory.


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Interaction Parameter Determination

In the last couple of years, the interest in the determination of predictive parameters within formulation development has grown. Interactions parameters provide valuable information for the prediction of molecular stability. These values are used in a “design of experiment” (DOE) approach to assist formulation developments. At ZentriForce Pharma we focus on the determination of molecule – molecule and molecule – formulation interactions. We could win the support of one of the leading scientists in the field of molecular interaction determination, (Professor Steve Harding of the NCMH in Nottingham, for our scientific advisory board. 

Determination of Molecule – Molecule Interactions

If the focus is solely on molecule – molecule interaction, the determination of the second osmotic virial coefficient (B22) via sedimentation equilibrium analytical ultracentrifugation (SE-AUC) is highly recommended. Alternatively, it is possible to determine the concentration dependence of the sedimentation coefficient (ks). Both, ks and B22 are in all cases positive as they solely describe the repulsive molecule – molecule interaction. We are able to provide high resolution and high throughput ks and B22 determinations  because we carry out all measurements on the new Optima Analytical Ultracentrifuge

Determination of Molecule – Formulation Interactions

If the aim of interaction parameter determination is to include the molecule –formulation interaction, the determination of the second osmotic virial coefficient (A22) via static light scattering (SLS) is recommended. Even though the name of this parameter is identical to B22, A22 describes additional interactions. Furthermore, the concentration dependence of the diffusion coefficient (kd) is assessable. Both, A22 and kd can be either positive or negative. They are mixtures of the repulsive molecule – molecule (always positive) and the molecule – formulation interaction (can be negative). With the DynaPro®Plate Reader III ZentriForce Pharma performs measurements on an instrument that was optimized for this purpose. We can provide both A22 and kd from a single experiment as we are able to simultaneously collect DLS and SLS data. 

Owing to a well-plate system the instrument allows high-throughput campaigns of different formulations, thus circumventing the time intensive procedure of a cuvette-based instrument. 

Minimum sample volume is 4 µl without the requirement to introduce an oil film to cover the sample.

Dynamic light scattering is known as a fast and efficient screening method for aggregate / particle detection. It is widely used within biopharmaceutical development. DLS closes the gap between techniques like size exclusion chromatography (SEC), that measure small soluble aggregates and particle methods such as light obscuration (LO) or micro flow imaging (MFI). Due to a high sensitivity toward large species, even small impurities can be detected within the measurement range (0.5 nm – 1 µm). Please follow the link for more information.  

Sedimentation velocity analytical ultracentrifugation (SV-AUC) is the gold standard in aggregation analytics. The importance of SV-AUC results is growing, especially in biosimilarity studies, and also within the development of gene therapy products.   ZentriForce Pharma offers SV-AUC services for a wide sample spectrum. For more information please follow the link.

Light scattering is a well defined effect. The physical properties of light scattering are used for several applications, such as dynamic and static light scattering. Please follow the link to a detailed description of light scattering theory. 

Sedimentation velocity analytical ultracentrifugation (SV-AUC) method is the established standard experiment when it comes to AUC. It is carried out at high rotational speed and the easiest way to obtain sample composition and species characteristics (e.g. molecular weight and frictional ratio). Follow this link to get more information about SV-AUC theory.

While sedimentation velocity is the standard AUC experiment, sedimentation equilibrium analytical ultracentrifugation (SE-AUC) nonetheless offers valuable information on an analyte.  SE-AUC is a first principle method for the determination of the molecular weight. Therefore, it is the method of choice to determine the molecular weight of your API in solution. Follow this link to get more information about SE-AUC theory.

Interested in Interaction parameter determination ?

We are more than happy to assist you developing your formulation.

DLS Specifications

Main OutputSize distribution (rH or dH); molecular weight (Mw)
Size range0.5 – 1000 nm; 1 kDa to 1 Mda
Aditional OutputsTemperature stability (Tonset, Tm, Tagg); Interaction parameter (A22, kD)
Sample Volume4 µL to 150 µL
Measurement typewell plate (96, 384 or 1536)
Temperature range 4°C to 85°C
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