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Zentriforce Pharma Research GmbH 2019 – all rights reserved
Sedimentation velocity analytical ultracentrifugation (SV-AUC) is the gold standard in aggregation analytics. The importance of SV-AUC results is growing, especially in biosimilarity studies, and also within the development of gene therapy products. ZentriForce Pharma offers SV-AUC services for a wide sample spectrum. For more information please follow the link.
Even though SV-AUC is with its more than 100 years of history a by far older technique than most others currently used within biopharmaceutical development. It is one of the most complex in regards of data evaluation. We have setup our own supercomputer cluster to be able to offer you several different data evaluation approaches, without the need of your data leaving our premises. We currently offer Sedfit and Ultrascan data evaluations. However, if we see the need to use a different software for your very molecule we have the knowledge base to do so. Please follow the link for more information.
While sedimentation velocity is the standard AUC experiment, sedimentation equilibrium analytical ultracentrifugation (SE-AUC) nonetheless offers valuable information on an analyte. SE-AUC is a first principle method for the determination of the molecular weight. Therefore, it is the method of choice to determine the molecular weight of your API in solution. Follow this link to get more information about SE-AUC theory.
Light scattering is a well defined effect. The physical properties of light scattering are used for several applications, such as dynamic and static light scattering. Please follow the link to a detailed description of light scattering theory.
With the increasing interest in gene therapy products also the interested in AF4 is growing. However, AF4 methods are comparably complex to develop. While most people use AF4 only as separation technique. We are experts in AF4 theory and use the theory to get more information out of the technique (e.g. particle size distributions). If you want to get more information about AF4 theory please follow this link.
ZentriForce Pharma was founded as a service provider that specializes in hydrodynamic techniques for biopharmaceutical development. The focus lies on gene therapy products and biosimilarity studies. Follow the link to our company page for further information.
ZentriForce Pharma implemented a strict quality system to assure the highest tractability of results generated at our facility. We have a wide range of quality related standard operation procedures (SOPS) in place to assure the consistency and quality of our laboratory operations. Please follow the link to a description of our quality system.
ZentriForce Pharma is your expert in analytical services, with focus on hydrodynamic techniques. Please follow the link to find out about our analytical services.
ZentriForce Pharma offers a wide range of analytical techniques. Please follow the link to our analytical portfolio.
ZentriForce Pharma is not merely an expert in analytical development. Our scientist are actively involved in further advances in the theory of hydrodynamic techniques. Follow the link to a detailed description of the theory behind our analytical services, including some of our own research
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Sedimentation velocity analytical ultracentrifugation (SV-AUC) method is the established standard experiment when it comes to AUC. It is carried out at high rotational speed and the easiest way to obtain sample composition and species characteristics (e.g. molecular weight and frictional ratio). SV-AUC measurements are comparably fast experiments. SV-AUC measurements may run between one hour and one day, depending on the solute’s size and the angular velocity. The maximum possible rotational speed is 60 krpm, which corresponds to 260,000 g. Depending on the chosen rotational speed, the size range of SV-AUC can be adapted to the requirements of the analyzed products. With the correct rotational speed settings, SV-AUC measurements can be applied to samples ranging from small peptides (1 kDa) to complete viruses (up to 200 nm).
Within a sedimentation velocity analytical ultracentrifugation experiment, the time dependent concentration distribution is measured. The sedimentation of solutes toward the cell bottom is monitored. The speed of sedimentation correlates with size, mass and shape of the monitored species. Modern softwares such as Ultrascan and Sefit use a finite element method approach to compute information such as molecular weight, partial concentration and the frictional ratio for all obtained species. For information about the differences between Sedfit and Ultrascan, please proceed to our AUC software page.
In an SV-AUC experiment the rotational speed is high enough to reduce the back diffusion of the solute to a low level. Peak broadening in SV-AUC is mostly caused by back diffusion along the concentration gradient caused by sedimentation. Even though modern algorithms such as Sedfit c(s) and Ultrascan PCSA apply a diffusion correction, a low level of back diffusion is essential for a high sensitivity.
Sedimentation velocity analytical ultracentrifugation (SV-AUC) is regarded as the gold standard in aggregation analytics. SV-AUC is a first principle method.
In contrast to size exclusion chromatography (SEC), it does not rely on a stationary phase. Interactions with the stationary phase might have an influence on the measured sample composition. It may, for example, specifically absorb species, split oligomers, or form such. Additionally, in SV-AUC, sample is not diluted with a mobile phase during the measurement. Therefore, SV-AUC can be used for the determination of the “real” aggregate/fragment analysis. For these reasons, regulatory bodies like FDA and EMA show steadily increasing interest in SV-AUC results. It is recommended to verify your SEC / AF4 results by SV-AUC measurements.
Sedimentation velocity analytical ultracentrifugation (SV-AUC) is the gold standard in aggregation analytics. The importance of SV-AUC results is growing, especially in biosimilarity studies, and also within the development of gene therapy products. ZentriForce Pharma offers SV-AUC services for a wide sample spectrum. For more information please follow the link.
Even though SV-AUC is with its more than 100 years of history a by far older technique than most others currently used within biopharmaceutical development. It is one of the most complex in regards of data evaluation. We have setup our own supercomputer cluster to be able to offer you several different data evaluation approaches, without the need of your data leaving our premises. We currently offer Sedfit and Ultrascan data evaluations. However, if we see the need to use a different software for your very molecule we have the knowledge base to do so. Please follow the link for more information.
While sedimentation velocity is the standard AUC experiment, sedimentation equilibrium analytical ultracentrifugation (SE-AUC) nonetheless offers valuable information on an analyte. SE-AUC is a first principle method for the determination of the molecular weight. Therefore, it is the method of choice to determine the molecular weight of your API in solution. Follow this link to get more information about SE-AUC theory.
Light scattering is a well defined effect. The physical properties of light scattering are used for several applications, such as dynamic and static light scattering. Please follow the link to a detailed description of light scattering theory.
With the increasing interest in gene therapy products also the interested in AF4 is growing. However, AF4 methods are comparably complex to develop. While most people use AF4 only as separation technique. We are experts in AF4 theory and use the theory to get more information out of the technique (e.g. particle size distributions). If you want to get more information about AF4 theory please follow this link.
Zentriforce Pharma Research GmbH 2019 – all rights reserved