SV-AUC Services for Virus Development

Related topics:

Sedimentation velocity analytical ultracentrifugation (SV-AUC) is the gold standard in aggregation analytics. The importance of SV-AUC results is growing, especially in biosimilarity studies, and also within the development of gene therapy products.   ZentriForce Pharma offers SV-AUC services for a wide sample spectrum. For more information please follow the link.

Asymmetrical flow field-flow fractionation (AF4), coupled with a multiangle laser light scattering detector (MALLS), has the potential to develop into a crucial technique for the evaluation of virus product purity. It is already one of the main assays in several cases of gene therapy product development. For further information please follow the link

Dynamic light scattering is an established analytical technique in the field of gene therapy product development. The size range of virus-based APIs (ca. 20-500 nm) lies well within the detection range of DLS (ca. 0.5- 1 μm). Dynamic light scattering is used to collect particle size distributions. The technique is robust and offers fast screening of large numbers of samples. For further information please follow the link

AUC is a first principle method. Therefore, the separation mechanism is well understood and extensively described. Please follow the link to a detailed description of AUC theory. 

Even though SV-AUC is with its more than 100 years of history a by far older technique than most others currently used within biopharmaceutical development. It is one of the most complex in regards of data evaluation. We have setup our own supercomputer cluster to be able to offer you several different data evaluation approaches, without the need of your data leaving our premises. We currently offer Sedfit and Ultrascan data evaluations. However, if we see the need to use a different software for your very molecule we have the knowledge base to do so. Please follow the link for more information. 

The detection of soluble aggregates is a crucial step for a wide range of bio-pharmaceutical products.The content of soluble aggregates is one of the most crucial quality attributes of pharmaceutical drug products. Their quantification is necessary for all drug products independent of the type of API. ZentriForce Pharma offers a wide range of analytical techniques for the detection of soluble aggregates.  If you need further information please follow the link

Depending on the size of your API, particle size determination (between 1 nm and 1 μm ) may be one of the most important steps for the monitoring of your drug product’s quality. ZentriForce Pharma offers a wide range of techniques to collect data on quality and stability properties of APIs. For further information please follow the link


General topics:

ZentriForce Pharma was founded as a service provider that specializes in hydrodynamic techniques for biopharmaceutical development. The focus lies on gene therapy products and biosimilarity studies. Follow the link to our company page for further information.

Please follow this link to our contact page

ZentriForce Pharma implemented a strict quality system to assure the highest tractability of results generated at our facility. We have a wide range of quality related standard operation procedures (SOPS) in place to assure the consistency and quality of our laboratory operations. Please follow the link to a description of our quality system. 

ZentriForce Pharma is your expert in analytical services, with focus on hydrodynamic techniques. Please follow the link to find out about our analytical services. 

ZentriForce Pharma offers a wide range of analytical techniques. Please follow the link to our analytical portfolio. 

ZentriForce Pharma is not merely an expert in analytical development. Our scientist are actively involved in further advances in the theory of hydrodynamic techniques. Follow the link to a detailed description of the theory behind our analytical services, including some of our own research

ZentriForce Pharma is always looking for motivated employees. Check out our career page for further information. 

Sedimentation Velocity Analytical Ultracentrifugation (SV-AUC) for Virus Development

Sedimentation velocity analytical ultracentrifugation (SV-AUC) has the potential to become one of the most crucial techniques for quality evaluation of gene therapy products.

Owing to their considerable size, intact viruses pose a challenge in analytics. However, our scientists have already tackled this problem before and use their experience to provide you with a custom-tailored method that is applicable to your complex samples.  

In addition to particle size distributions that give information on purity, SV-AUC can provide data on the nature of the observed species, including molecular weight, UV  260 nm/ 280 nm signal ratio, sedimentation coefficient and shape information. Its versatility makes SV-AUC one of the most powerful stability and quality indicating techniques in the field of gene therapy.

Please follow the link to our SV-AUC theory page to learn more about sedimentation velocity analytical ultracentrifugation (SV-AUC).

SV-AUC Services

We provide SV-AUC methods that combine highest quality results and maximum throughput of up to 14 samples per run.  Consequently, we will keep the cost of sample analysis as low as possible.

Cutting-edge instrumentation allows us to provide high resolution and high throughput at the same time. We carry out all measurements on the new Optima Analytical Ultracentrifuge. In addition, we offer high quality data evaluation approaches, such as Ultrascan, that require vast experience in the field of AUC data evaluation. Our own computer cluster allows us to carry out all data evaluation at our own facility.

The Optima Analytical Ultracentrifuge further enables us to collect two different UV Signals (e.g. 260 and 280 nm), a feature that is especially useful for species characterization of AAV samples.

Sedimentation velocity analytical ultracentrifugation (SV-AUC) is seen as the gold standard for aggregation analytics. The importance of SV-AUC results is growing especially in biosimilarity studies as well as the development of gene therapy products.   ZentriForce Pharma offers SV-AUC services for a wide range of samples. For more information please follow the link.

Asymmetrical flow field-flow fractonation (AF4), coupled with a multi-angle laser light scattering detector (MALLS) has the potential to develop into a crucial technique for the evaluation of virus product purity. It is already one of the main assays within several cases of gene therapy product development. For further information please follow the link

Dynamic light scattering is an established analytical technique in the field of gene therapy product development. The size range of virus-based APIs (ca. 20-500 nm) lies well within the detection range of DLS (ca. 0.5- 1 μm). Dynamic light scattering is used to collect particle size distributions. The technique is robust and the robustness of the technique and the possibility for a fast screening of large numbers of samples. For further information please follow the link

AUC is a first principle method. Therefore, the separation mechanism is well understood and extensively described. Please follow the link to a detailed description of AUC theory. 

Even though SV-AUC is with its more than 100 years of history a by far older technique than most others currently used within biopharmaceutical development. It is one of the most complex in regards of data evaluation. We have setup our own supercomputer cluster to be able to offer you several different data evaluation approaches, without the need of your data leaving our premises. We currently offer Sedfit and Ultrascan data evaluations. However, if we see the need to use a different software for your very molecule we have the knowledge base to do so. Please follow the link for more information. 

The detection of soluble aggregates is a crucial step for a wide range of bio-pharmaceutical products.The content of soluble aggregates is one of the most crucial quality attributes of pharmaceutical drug products. Their quantification is necessary for all drug products independent of the type of API. ZentriForce Pharma offers a wide range of analytical techniques for the detection of soluble aggregates.  If you need further information please follow the link

Depending on the size of your API, particle size determination (between 1 nm and 1 μm ) may be one of the most important steps for the monitoring of your drug product’s quality. ZentriForce Pharma offers a wide range of techniques to collect data on quality and stability properties of APIs. For further information please follow the link

Interested in AUC Services ?

We are more than happy to assist you with our AUC services.

Sedimentation Velocity Analytical Ultracentrifugation Specifications

Main outputSedimentation coefficient distribution (s, relative concentrations); molecular weight (Mw)
Size range0.5 – 200 nm; 0.5 kDa to n.a.* 
Additional outputsshape information (frictional ratio f/f0); Interaction parameter (B22, ks)
Sample volume400 µL 
Measurement type12 mm AUC measurement cells with Sapphire windows
Temperature range 4 °C to 40 °C

* The maximum size is restricted by the particle size

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