Dynamic Light Scattering (DLS) Services

Related topics:

Dynamic light scattering is known as a fast and efficient screening method for aggregate / particle detection. Therefore, it is widely used within biosimilarity studies to close the gap between results gained in the range of small soluble aggregates via size exclusion chromatography (SEC) and particle methods such as light obscuration (LO) and micro flow imaging (MFI). Due to the high sensitivity toward large species, even small impurities can be detected within the measurement range (0.5 nm – 1 µm). If you need further information please follow the link

DLS is a highly sensitive method for the detection particles. It is a fast and effective screening method for formulation development and production optimization. If you want to hear more about the use of DLS in biopharmaceutical development please follow the link.

Gene therapy is a comparably new trend in biopharmacuetical development. Therefore, there is not yet a fixed portfolio of standard analytical approaches. However, it is clear that DLS is one of these techniques. It is a fast and sensitive method for sizing of species in the sub-micron region.  Our scientists have already worked with a wide range of gene therapy products (ranging from small DNA/RNA complexes over adeno-associated viruses and lysosomal formulations up to complete viruses) and are keen to assist you with your very molecule. If you need more information please follow one of the following links.

Light scattering is a well defined effect. The physical properties of light scattering are used for several applications, such as dynamic and static light scattering. Please follow the link to a detailed description of light scattering theory. 

We are offering a wide range of analytical techniques for the determination of particle size distributions.  The detection of nano particle size distributions (1 nm  – 1 μm) is growing in importance. Especially, since the interest in gene therapy increased. Our scientist are working since more than 10 years on the topic of particle size determination. Please follow the link for more information.

Temperature stability testing is a crucial step in both biosimiliarity studies and formulation development. It is also a valuable tool in production optimization. With our setups we can support you either investigating the aggregation temperature or doing isotherm stability testings. If you need further information please follow the link

In the last couple of years, the interest in the determination of predictive parameters within formulation development has grown. Interactions parameters provide valuable information for the prediction of molecular stability. These values are used in a “design of experiment” (DOE) approach to assist formulation developments. Please follow the link for more information.


General topics:

ZentriForce Pharma was founded as a service provider that specializes in hydrodynamic techniques for biopharmaceutical development. The focus lies on gene therapy products and biosimilarity studies. Follow the link to our company page for further information.

Please follow this link to our contact page

ZentriForce Pharma implemented a strict quality system to assure the highest tractability of results generated at our facility. We have a wide range of quality related standard operation procedures (SOPS) in place to assure the consistency and quality of our laboratory operations. Please follow the link to a description of our quality system. 

ZentriForce Pharma is your expert in analytical services, with focus on hydrodynamic techniques. Please follow the link to find out about our analytical services. 

ZentriForce Pharma offers a wide range of analytical techniques. Please follow the link to our analytical portfolio. 

ZentriForce Pharma is not merely an expert in analytical development. Our scientist are actively involved in further advances in the theory of hydrodynamic techniques. Follow the link to a detailed description of the theory behind our analytical services, including some of our own research

ZentriForce Pharma is always looking for motivated employees. Check out our career page for further information. 

Want to talk to our DLS expert?

If you have any question regarding DLS Services get in touch with our DLS expert Dr. Marius Schmid

Dynamic Light Scattering (DLS) Services 

Zentriforce Pharma is a contract research organization that specializes in analytical services with a focus on hydrodynamic methods. We routinely conduct biosimilarity and gene therapy studies and are furthermore experienced in the development of dynamic light scattering (DLS) methods for a large variety of samples. ZentriForce Pharma offers DLS services under BSL 1 and 2.

Dynamic light scattering is known as a fast and sensitive screening method for aggregate / particle detection and therefore widely used within biopharmaceutical development. Owing to its large measurement range (0.5 nm – 1 µm) DLS closes the gap between the range of small soluble aggregates that are detected via size exclusion chromatography (SEC) and particles analyzed by methods such as light obscuration (LO) or micro flow imaging (MFI). Owing to the technique’s high sensitivity towards large species, even small impurities caused by aggregation can be detected

Please follow the link to our light scattering theory page to learn more about dynamic and static light scattering theory.

DLS Instrumentation

With the DynaPro®Plate Reader III, ZentriForce Pharma offers highest quality DLS measurements. The instrument performs dynamic light scattering (DLS) as well as static light scattering (SLS) measurements. Its properties allow for drastic reduction of sample volume, with a minimum of 4 µL, without the need to introduce an oil film to cover the sample.

Light Scattering (DLS+SLS) and UV/VIS Plate Reader Instrumentation used at ZentriForce Pharma.

Additionally, sample measurement at low concentrations is feasible, owing to the instrument’s high sensitivity. The instrument’s well-plate system allows high throughput campaigns. This allows us to assess the particle size distribution of different samples within a single measurement (e.g. within a formulation development).

DLS related Services

Our scientists have worked with a wide range of biopharmaceutical products and taken part in numerous biosimilarity studies.

Simultaneous DLS and SLS (static light scattering) measurements allow us to combine information on particle size distribution and molecular weight. This data provides valuable information on stability.An example for the benefit of this method is the simultaneous determination of the second osmotic virial coefficient (A22) and the concentration dependence of the diffusion coefficient (kD). The combination of these two parameters is especially interesting within formulation development studies.  For further information follow this link.

Dynamic Light Scattering and Static Light Scattering Experiment

We additionally offer temperature-controlled measurements with the options to either ramp the temperature to identify the aggregation temperature, or to monitor sample stability at a constant temperature. The well-plate-based design allows us to measure the samples simultaneously instead of consecutively (as is the case in injection cell-based setups). We are thus able to provide identical measurement conditions for all samples.  For further information, please follow this link to our temperature stability testing page.

Sample Specific DLS Services

Our scientists have already worked with a wide range of biopharmaceutical products. Our experience in DLS method development keeps your expenses low.

We provide detailed information for specific sample categories. Please follow the links in the related topics section on the left to find sample specific DLS information. 

If your sample type is not mentioned in the topics section, feel free to contact us directly

Dynamic light scattering is known as a fast and efficient screening method for aggregate / particle detection. Therefore, it is widely used within biosimilarity studies to close the gap between results gained in the range of small soluble aggregates via size exclusion chromatography (SEC) and particle methods such as light obscuration (LO) and micro flow imaging (MFI). Due to the high sensitivity toward large species, even small impurities can be detected within the measurement range (0.5 nm – 1 µm). If you need further information please follow the link

DLS is a highly sensitive method for the detection particles. It is a fast and effective screening method for formulation development and production optimization. If you want to hear more about the use of DLS in biopharmaceutical development please follow the link.

Gene therapy is a comparably new trend in biopharmacuetical development. Therefore, there is not yet a fixed portfolio of standard analytical approaches. However, it is clear that DLS is one of these techniques. It is a fast and sensitive method for sizing of species in the sub-micron region.  Our scientists have already worked with a wide range of gene therapy products (ranging from small DNA/RNA complexes over adeno-associated viruses and lysosomal formulations up to complete viruses) and are keen to assist you with your very molecule. If you need more information please follow one of the following links.

Light scattering is a well defined effect. The physical properties of light scattering are used for several applications, such as dynamic and static light scattering. Please follow the link to a detailed description of light scattering theory. 

We are offering a wide range of analytical techniques for the determination of particle size distributions.  The detection of nano particle size distributions (1 nm  – 1 μm) is growing in importance. Especially, since the interest in gene therapy increased. Our scientist are working since more than 10 years on the topic of particle size determination. Please follow the link for more information.

Temperature stability testing is a crucial step in both biosimiliarity studies and formulation development. It is also a valuable tool in production optimization. With our setups we can support you either investigating the aggregation temperature or doing isotherm stability testings. If you need further information please follow the link

Dynamic Light Scattering Specifications

Main outputSize distribution (rH or dH); molecular weight (Mw)
Size range0.5 – 1000 nm; 1 kDa to 1 MDa
Additional outputsTemperature stability (Tonset, Tm, Tagg); Interaction parameter (A22, kD)
Sample volume4 µL to 150 µL
Measurement typewell plate (96, 384 or 1536 wells)
Temperature range 4°C to 85°C
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